| Solution Information | help | |
| Enzyme: | C-C chemokine receptor type 6 | |
| inhibitor: | BDBM47823 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | A. Brief Description of the Assay: The purpose of this assay is to detect antagonists that inhibit the activation of the CCR6 receptor in the CHO-K1 beta-Arrestin Cell Line in 1536-well plate format in uHTS mode. B. Materials: PathHunter CHO-K1 CCR6 b-arrestin cell line (DiscoveRx, Cat# 93-0194C2) F12 nutrient mix HAMs (Invitrogen, Cat# 11765) Fetal Bovine Serum, heat-inactivated (Hyclone, Cat# SH30396) 100X Penicillin/Streptomycin Solution (Invitrogen, Cat#15140-122) Hygromycin B (Roche, Cat# 10843555001) Geneticin (MPBiomedicals, Cat # 1672548) Trypsin-EDTA 0.25% (Invitrogen, Cat# 25200-056) Cell Dissociation Buffer (Invitrogen, Cat# 13151) DPBS (Hyclone, Cat# 30028.02) T225 TC Flask (Nunc, Cat# 159934) 1536-well, white, solid-bottom, Kalypsys compatible, TC plate (Aurora) MIP-3a/CCL20 peptide (R&D Systems, Cat# 360MP) PathHunter Detection Reagents (DiscoveRx, Cat# 93-0001) Galacton Star Emerald 11 Cell Assay Buffer C. uHTS Procedures: Day1 Cell Seeding 1) Plate 800 cells/well i | |
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